Philipp Hoppe, PhD
(Co-Mentor: Dominic Hoepfner, PhD)
Chemical Biology & Therapeutics
Our group is integrated into a multidisciplinary research department, and we apply our knowledge to unravel novel disease intervention points. The main aim of our group is to identify potential new drug targets and the molecular pathways that they are involved in. The team has in-depth knowledge in genome-wide genetic approaches and sequencing technologies.
Our general focus is on mammalian systems, using mutagenesis in cell lines, ES/iPS cell-derived progeny and primary cells, with the application of CRISPR/Cas9-mediated gene editing approaches being one of our central methods. We are constantly improving our technical know-how around genome-editing assays, starting from simple survival-based, single knock-out screens up to multi-fate readouts of individual cells with simultaneous manipulation of several genes. In combination with flow cytometry and liquid handling equipment, we apply these tools to various disease models and are able to ask specific questions around a focused set of target genes or apply genome-wide methodologies.
We are mostly interested in research questions in the fields of DNA repair, immunology, regenerative medicine, and lineage choice of various stem cell systems, and we collaborate with other groups in these fields of expertise. Ultimately, we are trying to find molecules that allow us to manipulate cells in such a way that they contribute to attenuate or even cure diseases. We believe that combining latest state-of-the-art methods with relevant scientific questions will lead us to new interesting discoveries.
Network plasticity of pluripotency transcription factors in embryonic stem cells.
Filipczyk A, Marr C, Hastreiter S, Feigelman J, Schwarzfischer M, Hoppe PS, Loeffler D, Kokkaliaris KD, Endele M, Schauberger B, Hilsenbeck O, Skylaki S, Hasenauer J, Anastassiadis K, Theis FJ, Schroeder T.
Nat Cell Biol. 2015 Oct;17(10):1235-46.
Single-cell technologies sharpen up mammalian stem cell research.
Hoppe PS, Coutu DL, Schroeder T.
Nat Cell Biol. 2014 Oct;16(10):919-27.
Biallelic expression of nanog protein in mouse embryonic stem cells.
Filipczyk A, Gkatzis K, Fu J, Hoppe PS, Lickert H, Anastassiadis K, Schroeder T.
Cell Stem Cell. 2013 Jul 3;13(1):12-3.
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