Razvan Nutiu, PhD
Center for Proteomic Chemistry
Cambridge, Massachusetts, United States
Aberrant RNA expression, regulation, and function can lead to disease. Our research group works toward finding chemical tools to probe the structure and biological function of RNA. We aim to understand what are the favored RNA motifs, the favored chemical structures, and the basic rules that govern interactions between RNA or RNA-protein complexes and small molecules. At the same time, we are interested in finding links between RNA structure and biological function, and we develop novel approaches to modulate RNA function with small molecules. For instance, we are interested in influencing splicing choices, inhibiting miRNA biogenesis, and changing the affinity of RNA-protein interactions. Our approach is highly integrated and iterative, spanning a variety of technologies such as structural and biophysical approaches, biochemical and cell-based functional assays, high-throughput screening, next-generation sequencing, and computational modelling. We are collaborating extensively with other RNA-focused groups at Novartis and academic institutions, and together we build relevant knowledge to treat human disease.
Identifying mRNA sequence elements for target recognition by human Argonaute proteins.
Li J, Kim T, Nutiu R, Ray D, Hughes TR, Zhang Z.
Genome Res. 2014 May;24(5):775-85
Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument.
Nutiu R, Friedman RC, Luo S, Khrebtukova I, Silva D, Li R, Zhang L, Schroth GP, Burge CB.
Nat Biotechnol. 2011 Jun 26;29(7):659-64
Splice site strength-dependent activity and genetic buffering by poly-G runs.
Xiao X, Wang Z, Jang M, Nutiu R, Wang ET, Burge CB.
Nat Struct Mol Biol. 2009 Oct;16(10):1094-100
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